BinderVerse

Fastest sequence-to-function service in the world! Binders are expressed in vitro from a linear DNA template, so there is no cloning and no cell culture. The crude reaction is captured directly on the probe and titrated against the target in solution, and the amount captured is measured on the same instrument immediately before the assay. Because the binder is used without purification, a design that expresses poorly can still be ranked.

Don't get fooled by bivalent binding. Commercial targets are commonly supplied as Fc-fusions or other bivalent constructs. When the binder is held on the probe and a bivalent target flows over it, both arms of the target can engage at once, and the avidity contribution makes the measured affinity appear tighter than the intrinsic 1:1 value. This workflow reverses the geometry: the target is immobilized and the purified binder is the analyte, at a concentration measured on the assay instrument, so every interaction is monovalent and the reported K_D is the intrinsic affinity.

Cell-free expression straight from synthesised DNA — no cloning, no cells to grow. The binder is purified on both its Twin-Strep and His handles, captured on a Strep-Tactin XT probe, and titrated against the target in solution. Concentration is measured on the same instrument that runs the assay.

Cell-free expression straight from synthesised DNA — no cloning, no cells to grow. Every run comes back with a gel of the crude reaction; add purification and you can also have a melting temperature, an SEC trace and a concentration. Nothing is measured against a target, so what you get back is whether the design expressed, how much of it there is, and whether it holds together.

The binder is expressed as a full-length antibody in mammalian cells and used without purification: the crude supernatant is captured on the probe and titrated against the target in solution. Expression level therefore does not have to be high, or even known. This is the shortest of the mammalian routes and the least expensive per design, because nothing is purified and nothing is shipped. Culture scale is chosen under Protocol customization.

The binder is expressed as a full-length antibody, affinity-purified, and used as the analyte at a known concentration while the target is immobilized on the probe. A full-length antibody is itself bivalent, so holding it on the probe would measure avidity; putting it in solution against an immobilized target measures the intrinsic affinity of one arm. SDS-PAGE and SEC report purity and oligomeric state. Culture scale -- 1 mL or 30 mL -- is chosen under Protocol customization.

The binder is expressed as a full-length antibody and affinity-purified on a Protein A/G column. Its concentration is measured on the assay instrument, then it is captured on the probe and titrated against the target in solution. SDS-PAGE and SEC report purity and oligomeric state, and the purified antibody can be shipped to you. Culture scale is chosen under Protocol customization.

The binder is expressed as a Fab in mammalian cells and used without purification: the crude supernatant is captured on the probe and titrated against the target in solution. Expression level therefore does not have to be high, or even known. This is the shortest of the mammalian routes and the least expensive per design, because nothing is purified and nothing is shipped. Culture scale is chosen under Protocol customization.

The binder is expressed as a Fab, affinity-purified, and used as the analyte at a known concentration while the target is immobilized on the probe. Every binding event is therefore monovalent, and the affinity reported is intrinsic rather than avidity-inflated. SDS-PAGE and SEC report the purity and the oligomeric state of the material that was measured. Culture scale -- 1 mL or 30 mL -- is chosen under Protocol customization.

The binder is expressed as a Fab and purified on both of the affinity handles it carries, which removes truncated chains and incompletely assembled material. Its concentration is measured on the assay instrument, then it is captured on the probe and titrated against the target in solution. SDS-PAGE and SEC report purity and oligomeric state, and the purified binder can be shipped to you. Culture scale is chosen under Protocol customization.

Binder expressed on T cell surface as a CAR. T cell activation measured on ELISA or ELISPOT.